This study was conducted in November 2015 in a 268-bed community hospital with common hallway bathrooms shared by up to 8 patients. All samples were collected without a UVC device and in a shared bathroom and with a permanently installed, wall-mounted automated UVC (254-nm) device. The decontamination cycle initiates only after there have been 30 seconds of no motion detected by infrared sensors, after which a UVC cycle will run for 5 minutes. The device is located directly above the door and has an automatic shutoff safety feature that terminates the cycle if the bathroom door is opened during each run. Both the UVC-treated bathroom and control bathroom contains a 6.0 L per flush, wash down the toilet, and have similar room volumes (12.7 and11.7 m3, respectively). Air and surface samples were collected in each bathroom 5 minutes and 30 seconds after each patient use—a time that accounts for the decontamination cycle and permits comparison of the samples between each bathroom. A dual-headed SAS 360 bioaerosol sampler (Fig 1) set to collect 150 L of air over 50 seconds was used to permit the simultaneous collection of aerobic and anaerobic bacterial cultures. The Effect of UV Disinfection On Public Restroom Surfaces Part 23 The Effect of UV Disinfection On Public Restroom Surfaces anaerobic Brucella plates were immediately placed in a candle jar post sampling and subsequently incubated anaerobically at 37°C for 48 hours before counting bacterial colonies. The aerobic 5% sheep blood agar plates were incubated at 37°C for 24-48 hours before counting. Surface bacteria on the toilet seat and handwashing sink counter were sampled using 65-mm Replicate Organism Detection and Counting (RODAC) Contact Plates containing Trypticase soy agar, with a contact time of 10-12 seconds, and were incubated aerobically at 37°C overnight. Surface sample locations were matched between the 2 bathrooms, with samples taken at random locations on the counter, approximately 1.5 m away from the toilet, and on the top of the toilet seat. They collected a total of 66 air samples (32 aerobic and 34 anaerobic) and 64 surface samples (32 counter and 32 toilet seats). Mean bacterial concentrations were compared between the 2 bathrooms for each of the different sample types using Welch t-tests.